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rat bmscs osteogenic differentiation medium  (Procell Inc)

 
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    Procell Inc rat bmscs osteogenic differentiation medium
    Rat Bmscs Osteogenic Differentiation Medium, supplied by Procell Inc, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/rat+bmscs+osteogenic+differentiation+medium/pm41969062-313-43-49?v=Procell+Inc
    Average 86 stars, based on 1 article reviews
    rat bmscs osteogenic differentiation medium - by Bioz Stars, 2026-07
    86/100 stars

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    SLJGP promoted osteogenic differentiation of BMSCs. (A) The effects of different doses of SLJGP on the viability of BMSCs were detected by CCK-8. (B) After osteogenic differentiation induction, the effects of SLJGP on cell activity were detected by CCK-8. The expression levels of osteogenic factors ALP (C) , OPN (D) , BMP2 (E) , and RUNX2 (F) were determined by ELISA. (G) Alizarin red staining was used to observe the formation of calcium nodules. (H) Histogram of calcification percentage. * P < 0.05, ** P < 0.01, *** P < 0.001. NC: negative serum control group; SLJGP: drug-containing serum of SLJGP; ALP: alkaline phosphatase; OPN: osteopontin, BMP2: bone morphogenetic protein 2, RUNX2: runt-related transcription factor 2

    Journal: Journal of Orthopaedic Surgery and Research

    Article Title: Shuanglongjiegu pill promoted bone marrow mesenchymal stem cell osteogenic differentiation by regulating the miR-217/RUNX2 axis to activate Wnt/β-catenin pathway

    doi: 10.1186/s13018-024-05085-0

    Figure Lengend Snippet: SLJGP promoted osteogenic differentiation of BMSCs. (A) The effects of different doses of SLJGP on the viability of BMSCs were detected by CCK-8. (B) After osteogenic differentiation induction, the effects of SLJGP on cell activity were detected by CCK-8. The expression levels of osteogenic factors ALP (C) , OPN (D) , BMP2 (E) , and RUNX2 (F) were determined by ELISA. (G) Alizarin red staining was used to observe the formation of calcium nodules. (H) Histogram of calcification percentage. * P < 0.05, ** P < 0.01, *** P < 0.001. NC: negative serum control group; SLJGP: drug-containing serum of SLJGP; ALP: alkaline phosphatase; OPN: osteopontin, BMP2: bone morphogenetic protein 2, RUNX2: runt-related transcription factor 2

    Article Snippet: Osteogenic differentiation was induced by rat BMSCs osteogenic induction differentiation medium (PD-008, Procell, China).

    Techniques: CCK-8 Assay, Activity Assay, Expressing, Enzyme-linked Immunosorbent Assay, Staining, Control

    SLJGP inhibited the expression of miR-217, which directly targeted RUNX2. (A) The expression of miR-217 in SLJGP-treated BMSCs was detected by RT-qPCR. (B) The binding relationship between miR-217 and RUNX2 was predicted by the targetscan website. (C) Dual-luciferase report assay verified the binding relationship between miR-217 and RUNX2. * P < 0.05, ** P < 0.01, *** P < 0.001. SLJGP: drug-containing serum of SLJGP

    Journal: Journal of Orthopaedic Surgery and Research

    Article Title: Shuanglongjiegu pill promoted bone marrow mesenchymal stem cell osteogenic differentiation by regulating the miR-217/RUNX2 axis to activate Wnt/β-catenin pathway

    doi: 10.1186/s13018-024-05085-0

    Figure Lengend Snippet: SLJGP inhibited the expression of miR-217, which directly targeted RUNX2. (A) The expression of miR-217 in SLJGP-treated BMSCs was detected by RT-qPCR. (B) The binding relationship between miR-217 and RUNX2 was predicted by the targetscan website. (C) Dual-luciferase report assay verified the binding relationship between miR-217 and RUNX2. * P < 0.05, ** P < 0.01, *** P < 0.001. SLJGP: drug-containing serum of SLJGP

    Article Snippet: Osteogenic differentiation was induced by rat BMSCs osteogenic induction differentiation medium (PD-008, Procell, China).

    Techniques: Expressing, Quantitative RT-PCR, Binding Assay, Luciferase

    SLJGP regulated miR-217/RUNX2 to promote osteogenic differentiation of BMSCs. (A) The expression level of miR-217 was detected by RT-qPCR. (B) The expression level of RUNX2 was detected by RT-qPCR. (C) CCK-8 was used to detect the cell activity in each group. (D-F) ELISA was used to detect the expression levels of ALP, OPN, and BMP2. (G) Alizarin red staining was used to observe the formation of calcium nodules. (H) Histogram of calcification percentage. * P < 0.05, ** P < 0.01, *** P < 0.001. SLJGP: drug-containing serum of SLJGP; ALP: alkaline phosphatase; OPN: osteopontin, BMP2: bone morphogenetic protein 2, RUNX2: runt-related transcription factor 2

    Journal: Journal of Orthopaedic Surgery and Research

    Article Title: Shuanglongjiegu pill promoted bone marrow mesenchymal stem cell osteogenic differentiation by regulating the miR-217/RUNX2 axis to activate Wnt/β-catenin pathway

    doi: 10.1186/s13018-024-05085-0

    Figure Lengend Snippet: SLJGP regulated miR-217/RUNX2 to promote osteogenic differentiation of BMSCs. (A) The expression level of miR-217 was detected by RT-qPCR. (B) The expression level of RUNX2 was detected by RT-qPCR. (C) CCK-8 was used to detect the cell activity in each group. (D-F) ELISA was used to detect the expression levels of ALP, OPN, and BMP2. (G) Alizarin red staining was used to observe the formation of calcium nodules. (H) Histogram of calcification percentage. * P < 0.05, ** P < 0.01, *** P < 0.001. SLJGP: drug-containing serum of SLJGP; ALP: alkaline phosphatase; OPN: osteopontin, BMP2: bone morphogenetic protein 2, RUNX2: runt-related transcription factor 2

    Article Snippet: Osteogenic differentiation was induced by rat BMSCs osteogenic induction differentiation medium (PD-008, Procell, China).

    Techniques: Expressing, Quantitative RT-PCR, CCK-8 Assay, Activity Assay, Enzyme-linked Immunosorbent Assay, Staining

    SLJGP regulated the miR-217/RUNX2 axis to activate the Wnt/β-catenin pathway in BMSCs. (A) The expressions of Wnt1, β-catenin, and RUNX2 were detected by WB. (B) Relative protein expression levels of Wnt1. (C) Relative protein expression levels of β-catenin. (D) Relative protein expression levels of RUNX2. (E) Immunofluorescence detection of β-catenin. (F) Mean fluorescence intensity of β-catenin. * P < 0.05, ** P < 0.01, *** P < 0.001. SLJGP: drug-containing serum of SLJGP; RUNX2: runt-related transcription factor 2

    Journal: Journal of Orthopaedic Surgery and Research

    Article Title: Shuanglongjiegu pill promoted bone marrow mesenchymal stem cell osteogenic differentiation by regulating the miR-217/RUNX2 axis to activate Wnt/β-catenin pathway

    doi: 10.1186/s13018-024-05085-0

    Figure Lengend Snippet: SLJGP regulated the miR-217/RUNX2 axis to activate the Wnt/β-catenin pathway in BMSCs. (A) The expressions of Wnt1, β-catenin, and RUNX2 were detected by WB. (B) Relative protein expression levels of Wnt1. (C) Relative protein expression levels of β-catenin. (D) Relative protein expression levels of RUNX2. (E) Immunofluorescence detection of β-catenin. (F) Mean fluorescence intensity of β-catenin. * P < 0.05, ** P < 0.01, *** P < 0.001. SLJGP: drug-containing serum of SLJGP; RUNX2: runt-related transcription factor 2

    Article Snippet: Osteogenic differentiation was induced by rat BMSCs osteogenic induction differentiation medium (PD-008, Procell, China).

    Techniques: Expressing, Immunofluorescence, Fluorescence